Coupling Factor 6 (CF6)

An endogenous vasoconstrictor suppresses the synthesis of prostacyclin.

Coupling Factor 6 Antibody for Immunohistochemistry (Catalog No.: H-007-53, $450/50 µl)

Protocol for Coupling Factor 6 Immunohistochemistry
Tissue Sample	Rat Heart
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Pronase
Blocking	2% Normal Goat Serum
Primary Antibody	Anti-Coupling Factor 6 (Human) serum (Catalog No.:H-007-53)
Optimal Dilution	1: 100
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector)
Detection system	HRP
substrate	DAB (Sigma)
Counterstained	Hematoxylin

Coupling Factor 6 Precursor (55-108) (Human), RIA Kit (RK-007-54)

Cross Reactivity

Peptide	% CR
Coupling Factor 6 Precursor (55-108)-NH2 (Human)	100
Coupling Factor 6 (33-108)-NH2 (Human)	100
Coupling Factor 6 (55-108)-NH2 (Rat)	0.66
Angiotensin II (Human)	0
Endothelin-1	0
Neuropeptide Y (NPY) (Human, Rat)	0
Urotensin II (Human)	0
Urotensin II (Rat)	0
CGRP (Human)	0
Adrenomedullin (1-52) (Human)	0

Normal plasma level of CF6 Precursor (55-108) in healthy subjects: 152 ± 16 pg/ml ( Mean ± SE, n=16) (non-extracted sample).

Coupling Factor 6 (Rat) Western Blot Kit

WBK-007-49

$600/kit

Osanai T., et al. J Clin Invest, October 2001, Volume 108, Number 7, 1023-1030