Ghrelin Kits - more information

Ghrelin RIA
More information

Ghrelin Level
Fasting plasma Ghrelin (Human)	514+/-63 pg/ml n=24	Otto B, et al. Eur J Endocrinol 2001 Nov;145(5):669-73
Fasting plasma Ghrelin (Human)	1057+/-95 pg/ml (anorectic patients	Otto B, et al. Eur J Endocrinol 2001 Nov;145(5):669-73
Ghrelin (Human)	307 +/- 164 [Prader-Willi syndrome (PWS)]	DelParigi A., et al. J Clin Endocrinol Metab 2002 Dec;87(12):5461-4
Ghrelin (Human)	109 +/- 24 fmol/ml	DelParigi A., et al. J Clin Endocrinol Metab 2002 Dec;87(12):5461-4
Fasting serum ghrelin levels (Human)	270 +/- 102 pmol/liter	Haqq AM, et al. J Clin Endocrinol Metab 2003 Jan;88(1):174-8
Fasting serum ghrelin levels (Human)	429 +/- 374 pmol/liter [Prader-Willi Syndrome (PWS)]	Haqq AM, et al. J Clin Endocrinol Metab 2003 Jan;88(1):174-8
Fasting serum ghrelin levels (Human)	139 +/- 70 pmol/liter (Obese group)	Haqq AM, et al. J Clin Endocrinol Metab 2003 Jan;88(1):174-8
Fasting plasma ghrelin	87+/-28 pmol/liter (Pima Indians)	Tschop M, et al. Diabetes 2001 Apr;50(4):707-9
Fasting plasma ghrelin	129+/-34 fmol/ml (Caucasians)	Tschop M, et al. Diabetes 2001 Apr;50(4):707-9
fasting healthy lean children (NC)	426.0; 183.0-618.0 pg/ml	Bellone S., et al. J Endocrinol Invest 2002 May;25(5):RC13-5
fasting obese children (OBC)	229.5; 162.5-339.5 pg/ml	Bellone S., et al. J Endocrinol Invest 2002 May;25(5):RC13-5
Fastin healthy lean adults (NA)	380.5; 257.7-551.7 pg/ml	Bellone S., et al. J Endocrinol Invest 2002 May;25(5):RC13-5

Circulating Ghrelin Levels Are Decreased in Human Obesity
	Lean Caucasians	Obese Caucasians	Lean Pima Indians	Obese Pima Indians
n	7	8	7	8
Sex-Female	3	4	3	4
Sex-Male	4	4	4	4
Age (Years)	32 ± 11	30 ± 7	33 ± 4	32 ± 5
Body weight (kg)	71.4 ± 9.8	109.8 ± 16.8	70.3 ± 9.6	109 ± 15.2
BMI (kg/m2)	25.4 ± 2.3	38.2 ± 4.8	24.0 ± 1.9	37.9 ± 6.6
Body fat (%)	22 ± 9	35 ± 7	24 ± 7	35 ± 5
Plasma Ghrelin (fmol/ml)	155 ± 25	106 ± 23	95 ± 13	80 ± 36
Plasma Glucose (mg/dl)	87 ± 7	91 ± 4	89 ± 6	95 ± 7
Plasma Insulin (uU/ml)	4 ± 3	10 ± 3	6 ± 3	18 ± 10
Plasma Leptin (ng/ml)	8 ± 7	53 ± 47	10 ± 9	35 ± 27
Tschšp, M., et al. Diabetes 50, 707-709, 2001

EK-031-30

RK-031-30

Extraction of plasma sample is not necessary with Phoenix's

human Ghrelin RIA Kit

Time of Use* Week 0 Week 2 Week 4

1 x 5.75pg (57.5pg/ml) 12.3pg (123pg/ml) 7.65pg (76.5pg/ml)

1/2 x 1.88pg 6.53pg 3.41pg

1/4 x 2.59pg 1.61pg

1/8 x 0.98pg 0.58pg

* Kits used in Week 0 were used within 2 days of manufacture. All kits used in Week 2 and 4 were stored as dry power (not reconstituted) at 4¡C.

**100 µl of plasma (non-extracted, normal human subjects) was used to perform the RIA. To calculate the level of Ghrelin-like immunoreactivity, the level obtained by extrapolation of the standard curve must be multiplied by 10 to obtain the level at 1 ml (ex. 12.3 pg x 10 = 123 pg/ml).

Stability of Ghrelin (Human) RIA Kit

(at least 3 weeks in liquid form)

Time of Rehydrate	B/T (%)	R/T (%)	ED₂₀ (pg/tube)	ED₅₀ (pg/tube)	ED₈₀ (pg/tube)
hour 1	0.04	0.44	23.78	8.74	3.14
Day 2	0.04	0.52	14.19	5.51	2.19
Week 1	0.05	0.50	23.72	8.18	2.65
Week 2	0.03	0.43	38.21	11.88	4.57
Week 3	0.03	0.44	31.82	11.12	3.64
Rehydrate one kit contents with RIA buffer keep them at 4¡C. Kits was used in above scheduled time.

Stability of Ghrelin (Human) RIA Kit

(at least 4 weeks in dry form)

Time of Use*	B/T (%)	R/T (%)	ED₂₀(pg/tune)	ED₅₀(pg/tune)	ED₈₀(pg/tune)
Week 0	0.05	0.37	20.90	5.78	1.99
Week 2	0.03	0.45	19.03	8.25	3.14
Week 4	0.03	0.35	15.75	6.28	2.27

* Kits used in Week 0 were used within 2 days of manufacture. All kits used in Week 2 and 4 were stored as dry power (not reconstituted) at 4¡C.

Phoenix strongly recommends using the RIA kit as soon as possible. Always store the unused kit as dry power at 4¡C if not used immediately. Never store reconstituted kit components as they are unstable. Use of stored reconstituted kit components to perform RIA may yield poor and unacceptable results.

Phoenix's Ghrelin (Human) RIA Kit detects "total" Ghrelin, including Ser³- octanoyl and Ser³-des-octanoyl Ghrelins.