NERP-2 (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat/Mouse Brain Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody (pre-immuno serum)
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-NERP-2 (Human) Antibody (Catalog No.:H-076-91)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-076-91

Family

NERP

Standard Size

100 µl

Sequence

pGlu - Ala - Glu - Ala - Thr - Arg - Gln - Ala - Ala - Ala - Gln - Glu - Glu - Arg - Leu - Ala - Asp - Leu - Ala - Ser - Asp - Leu - Leu - Leu - Gln - Tyr - Leu - Leu - Gln - Gly - Gly - Ala - Arg - Gln - Arg - Gly - Leu - Gly - NH2

Species

Human

Host

Rabbit

Immunogen

human NERP-2

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry - 1:500

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