GLP-1 & GLP-2

Normal Plasma Peptide/Protein Levels

Neuron Function Modulation

Protocol	Fluorescent EIA Kit (PDF) *Please Note: For best results, use DPP-IV instead of Aprotinin.
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-028-11
Standard Size	96 well plate
Sensitivity	Minimum Detectable Concentration = 27.6 pg/ml
Range	0-10000 pg/ml
Linear Range	27.6-676 pg/ml
Cross Reactivity	Peptide %Cross-reactivity GLP-1 (7-36) (Human, Rat, Mouse) 100 GLP-1 (9-36) Amide 100 GLP-1 (1-36) Amide (Human) 100 GLP-1 (7-37) (Human, Rat, Mouse) 0.4 GLP-2 (Human) 0 Glucagon (Human, Rat, Mouse, Porcine, Bovine) 0 CGRP 0 GIP 0 VIP 0 Insulin 0 Secretin 0 Exendin-4 0
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	10.3-747 pg/ml
ED50	52.64 pg/ml
Sample Level	26.75 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.