Secreted Frizzled-related Protein 2 / SFRP 2 (25-50) (Human)

Image

Protocol for Immunohistochemistry:
Tissue Sample	Rat/Mouse Visceral Fat
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-sFRP-2 (25-50) Antiserum (Catalog No: H-006-10)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-006-10

Family

sFRP

Standard Size

100 µl

Sequence

Gln - Gly - Gly - Glu - Leu - Val - Ile - Thr - Ser - Val - Lys - Arg - Trp - Gln - Lys - Gly - Gln - Arg - Glu - Phe - Lys - Arg - Ile - Ser - Arg - Ser - Ile - Arg - Lys - Leu - Gln - Cys

Species

Human

Host

rabbit

Immunogen

sFRP-2 (25-50) (Human)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_s_