Leptin

Leptin Sequence Comparison

Catalog #	003-17
Standard Size	200 µg
Sequence	Human Leptin is 146 Amino Acid residues protein cloned, expressed in E.Coli and purified. The purity of Leptin is >97% as determined by SDS - PAGE. The identity of the protein has been confirmed by N - terminal sequence analysis. Endotoxin level in the final preparation is <1ng/µg of Cytokinin (By LAL method).
Species	Rat
M.W	~16 kDa
Solubility	Insoluble in water. Dissolve peptide in 0.5 ml/1 mg 15 mM HCl, after Protein is completely dissolved, add 0.3 ml/1 mg 7.5 mM NaOH to bring pH up approximately to 5.2.
Storage Condition	Store peptide in DRY form at -20°C.
Appearance	White powder
Content	The contents of this vial have been accurately determined to contain 200µg of NET peptide.
HPLC Analysis	The purity of Leptin is >97% as determined by SDS-PAGE.

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For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.