Relaxin & Relaxin-Related Peptides

Insulin-like 6 Peptide (INSL 6)

Relaxin Evolution

GSS: Relaxin Like Peptide

Antibody Production & Evaluation

Image	Protocol for Immunohistochemistry:   Tissue Sample  Human testis  Fixative  10% Formalin  Embedding  Paraffin  Negative control  No primary antibody  Pretreatment  Target retrieval 25 min (Steam)  Blocking  2% Normal Goat Serum  Primary Antibody Rabbit Anti-INSL 6 (Human) Antiserum (Catalog No.:H-035-30)  Optimal Dilution  1:2000 (1hour at RT)  Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (Vector) (1:400), 30 min  Amplification  ABC (Vector) (1:400, 30 min)  Detection system  HRP  Substrate  DAB (Sigma)  Counterstained  Hematoxylin, 30 sec
Catalog #	H-035-30
Standard Size	50 µl
Species	Human
Storage Condition	Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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