Autotaxin (369-391) (Human, Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat & Mouse Kidney, Rat Liver
Fixative	10% Formalin
Embedding	Paraffin
Negative control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Autotaxin (369-399) (Human, Mouse) Serum (Catalog No.:H-008-29)
Optimal Dilution	1:250~500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin

Catalog #

H-008-29

Standard Size

100 µl

Sequence

Thr - Glu - Phe - Leu - Ser - Asn - Tyr - Leu - Thr - Asn - Val - Asp - Asp - Ile - Thr - Leu - Val - Pro - Gly - Thr - Leu - Gly - Arg

Species

Human, Mouse

Reconstitution

Reconstitute with 100 ul distill water.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.

Recommended Dilution Factor

Indirect ELISA: 1: 6400
Immunohistochemistry:1: 250~ 500

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