Urocortin II (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample Human pancreas tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Urocortin II (Human) Antiserum (Catalog No.:H-019-30)
Optimal Dilution 1:500 (overnight at 4^oC)
Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
Substrate DAB (Sigma), 10"
Counterstained Hematoxylin, 30 Sec

Catalog #

H-019-30

Standard Size

50 µl

Sequence

Ile - Val - Leu - Ser - Leu - Asp - Val - Pro - Ile - Gly - Leu - Leu - Gln - Ile - Leu - Leu - Glu - Gln - Ala - Arg - Ala - Arg - Ala - Ala - Arg - Glu - Gln - Ala - Thr - Thr - Asn - Ala - Arg - Ile - Leu - Ala - Arg - Val - NH2

Species

Human

Host

Rabbit

Immunogen

Urocortin II (Human)/ SRP (6-43)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- Urocortin II (Human) Serum in the lyophilized form. The HPLC purified standard peptide, Urocortin II (Human) can be detected with this antiserum at 1: 12800 (0.078μl/ml) in ELISA.

Recommended Dilution Factor

Direct ELISA: 1: 3200
Immunohistochemistry: 1:500

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Xu et al. Expression and Role of the Corticotropin-Releasing Hormone/Urocortin-Receptor-Binding Protein System in the Primate Corpus Luteum during the Menstrual Cycle
Endocrinology. 2007 Nov;148(11):5385-95.

Karteris et al. Urocortin II is expressed in human pregnant myometrial cells and regulates myosin light chain phosphorylation: potential role of the type-2 corticotropin-releasing hormone receptor in the control of myometrial contractility.
Endocrinology. 2004 Feb;145(2):890-900.