ANP (Atrial Natriuretic Peptide)

Normal Plasma Peptide/Protein Levels

Antibody Production & Evaluation

Image	Protocol for Immunohistochemistry:  Tissue Sample   Rat and Mouse heart tissues  Fixative  10% Formalin  Embedding  Paraffin  Negative control  No primary antibody  Pretreatment  N/A  Blocking  3% H2O2, 2% Normal Goat Serum  Primary Antibody  Rabbit anti-ANP, Prepro (26-55) (H) antibody (Cat. No.: H-005-19)  Optimal Dilution  1:500  Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)  Amplification  Streptavidin-HRP (Vector), 1:400, 30 min  Detection system  HRP  Substrate  DAB (sigma), 30min  Counterstained  Hematoxylin, 30Sec
Catalog #	H-005-19
Family	LANP
Standard Size	50 µl
Sequence	Asn - Pro - Met - Tyr - Asn - Ala - Val - Ser - Asn - Ala - Asp - Leu - Met - Asp - Phe - Lys - Asn - Leu - Leu - Asp - His - Leu - Glu - Glu - Lys - Met - Pro - Leu - Glu - Asp -
Species	Human
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage Condition	Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content	This vial contains 50µl of rabbit anti- prepro-ANF (26-55) (Human) serum in the lyophilized form.
Recommended Dilution Factor	Immunofluorescence: 1:200 ABC: 1:500
Cross Reactivity	Peptide % Crossreactivity LANP/ANP (26-55), prepro (Human) 100 Alpha-ANP (1-28) (Human, Canine, Porcine, Bovine) 0 ANP (Rat) 0 Beta-ANP (Human) 0 Prepro-ANP (56-92) (Human) 0 BNP-32 (Human) 0 CNP-22 (Human, Porcine, Rat) 0 Insulin 0 IGF-1 0

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this peptide:

Lundberg et al. Natriuretic peptide expression in the heart of the TTR-ANP transgenic mouse-Comparison to the normal heart.
Microsc Res Tech. 2005 Oct;68(2):97-106.