Visfatin (412-431) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat Liver
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody (pre-immuno serum)
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Visfatin (412-431) (Human) Antiserum (Catalog No.:H-003-82)
Optimal Dilution	1:200-500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-003-82

Standard Size

100 µl

Sequence

Asn - Val - Phe - Lys - Asp - Pro - Val - Ala - Asp - Pro - Asn - Lys - Arg - Ser - Lys - Lys - Gly - Arg - Leu - Ser

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of Rabbit Anti- Visfatin (412-431) (Human) Serum in the lyophilized form.

Recommended Dilution Factor

Immunohistochemistry (ABC): 1: 250~500
Western blot analysis: 1: 250~500

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