Rat hypothalamus tissue was stained byOrexin A (Human, Bovine, Mouse, Rat) Antiserum
Protocol for Immunohistochemistry
Fixative
10% Formalin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti- Orexin A (Human, Bovine, Mouse, Rat) (Catalog No.:H-003-30)
Optimal Dilution
1:2000 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Links to publications that use this antibody:
Rolf Fronczek, Sarita van Geest, Marijke Frölich, et al, Hypocretin (orexin) loss in Alzheimer's disease.Neurobiol Aging. 2012 Aug;33(8):1642-50. doi: 10.1016/j.neurobiolaging.2011.03.014. Epub 2011 May 5.
Ettruo et al. The anatomy of the Göttingen minipig hypothalamus. J Chem Neuroanat. 2010 May;39(3):151-65. Epub 2009 Dec 28.
Solomon et al. Peripheral ghrelin interacts with orexin neurons in glucostatic signalling.Regul Pept. 2007 Dec 4;144(1-3):17-24.
Fronczek et al. Hypocretin (orexin) loss in Parkinson’s disease Brain. 2007 Jun;130(Pt 6):1577-85.
Hamlin et al. The neural correlates and role of D1 dopamine receptors in renewal of extinguished alcohol-seeking. Neuroscience. 2007 May 11;146(2):525-36.
Satoh et al. Expression pattern of FOS in orexin neurons during sleep induced by an adenosine A2A receptor agonist. Behav Brain Res. 2006 Jun 30;170(2):277-86.
Helwig et al. PC1/3 and PC2 gene expression and post-translational endoproteolytic pro-opiomelanocortin processing is regulated by photoperiod in the seasonal Siberian hamster (Phodopus sungorus). J Neuroendocrinol. 2006 Jun;18(6):413-25.
Overeem et al. Immunohistochemical screening for autoantibodies against lateral hypothalamic neurons in human narcolepsy. J Neuroimmunol. 2006 May;174(1-2):187-91.
Fronczek et al. The number of hypothalamic hypocretin (orexin) neurons is not affected in Prader-Willi syndrome.J Clin Endocrinol Metab. 2005 Sep;90(9):5466-70.
Karteris et al. Food deprivation differentially modulates orexin receptor expression and signaling in rat hypothalamus and adrenal cortex.Am J Physiol Endocrinol Metab. 2005 Jun;288(6):E1089-100.
Petersen et al. Orexin loss in Huntington's disease. Hum Mol Genet. 2005 Jan 1;14(1):39-47.
Satoh et al. FOS expression in orexin neurons following muscimol perfusion of preoptic area.Neuroreport. 2004 May 19;15(7):1127-31.
Toshinai et al. Ghrelin-Induced Food Intake Is Mediated via the Orexin Pathway Endocrinology. 2003 Apr;144(4):1506-12.
Kohsaka et al. A significant participation of orexin-A, a potent orexigenic peptide, in the preovulatory luteinizing hormone and prolactin surges in the rat.Brain Res. 2001 Apr 13;898(1):166-70. Chen et al. Orexin A-like immunoreactivity in the rat brain.Neurosci Lett. 1999 Feb 5;260(3):161-4.
