Leptin (Rat) - Antibody for Immunohistochemistry

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Western Blot Analysis of Vaspin in rat visceral white adipose tissue
Tissue Sample	Rat visceral white adipose tissue homogenate
Electrophoresis	15% Tricine-PAGE gel
Negative control	No primary antibody
Blocking	1% BSA in TBST or 5% dry milk in TBST
Primary Antibody	Rabbit Anti-Leptin (Rat) Antiserum (Catalog No.:H-003-17)
Optimal Dilution	1:500-~1000 (4 hour at RT, or overnight at 4° C)
Secondary Antibody	Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system	HRP
Substrate	SuperSignal Chemiluminescent Substrate

Protocol for Immunohistochemistry
Tissue Sample	Mouse and Rat visceral white adipose tissue homogenate
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Leptin ( Rat) Serum (Catalog No.:H-003-17)
Optimal Dilution	1:500~1000 (1 hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-003-17

Standard Size

100 µl

Species

Rat

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

The contents of this vial have been accurately determined to contain 100µl of rabbit anti-Leptin (Rat) (Recombinant) serum in the lyophilized form

Recommended Dilution Factor

1: 500 ~ 1000 (western blot)
1: 12800 (indirect ELISA)
1: 500~1000 (Immunohistochemistry-ABC)

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