FGF-21 (26-47) (Human) - Antibody for Immunohistochemistry

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Procotol for Immunohistochemistry
Tissue Sample	Human/Mouse liver tissue & Human hepatocellular carcinoma tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-FGF-21 (26-47) (Human) Serum (Catalog No.:H-002-46)
Optimal Dilution	1:250~500 (over night at 4 ^OC)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Western blot analysis
Sample Rat liver tissue
Electrophoresis 15% SDS-PAGE gel, reducing condition
Denatured sample 100'C for 5 min
Positive control recombinant human FGF-21
Blocking 1% BSA in TBST
Primary Antibody Rabbit Anti-FGF-21 (26-47) (Human) Serum (Catalog No.:H-002-46)
Optimal Dilution 1:500 (overnight at cold room)
Secondary Antibody Rabbit anti-Mouse IgG, HRP conjugated
Substrate ECL
Positive Band ~ 23 KDa

Catalog #

H-002-46

Standard Size

100 µl

Sequence

Cys - Gln - Ala - His - Pro - Ile - Pro - Asp - Ser - Ser - Pro - Leu - Leu - Gln - Phe - Gly - Gly - Gln - Val - Arg - Gln - Arg

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of rabbit anti-FGF-21 (26-47) (Human) serum in the lyophilized form.

Recommended Dilution Factor

Direct ELISA: 1:6400
Immunohistochemistry (ABC): 1:250-500
Western blot: 1:350-500

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