GPR-54 (375-396) (Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat hypothalamus tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-GRP54 (375-396) (Mouse) Serum (Catalog No.:H-001-64)
Optimal Dilution	1:50-100 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min

Catalog #

H-001-64

Standard Size

100 µl

Sequence

Thr - Pro - Glu - Pro - Gly - Asn - Pro - Val - Val - His - Ser - Pro - Ser - Val - Gln - Asp - Glu - His - Thr - Ala - Pro - Leu

Species

Mouse

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of rabbit anti-GPR54 (375-396) (Mouse) serum in the lyophilized form. The HPLC purified standard peptide, GPR54 (375-396) (Mouse) can be detected with this antiserum (1: 6400, 0.156ug/ml) in ELISA system.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Richard et al. KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo by oestradiol and gonadotrophin-releasing hormone.
J Neuroendocrinol. 2008 Mar;20(3):381-93.

Mead et al. Kisspeptins Are Novel Potent Vasoconstrictors in Humans, with a Discrete Localization of Their Receptor, G Protein- Coupled Receptor 54, to Atherosclerosis-Prone Vessels
Endocrinology. 2007 Jan;148(1):140-7.

Castellano et al. Expression of KiSS-1 in rat ovary: putative local regulator of ovulation?
Endocrinology. 2006 Oct;147(10):4852-62.