Adipo R1 (357-375) / Adiponectin Receptor Type 1 (357-375) (Human)

Image

Protocol for Immunohistochemistry
Tissue Sample	Rat Heart tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Adiponectin Receptor 1 (357-375) (Human) Serum (Catalog No.:H-001-44)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-001-44

Standard Size

50 µl

Sequence

Asn - Leu - Gln - Glu - Phe - Arg - Tyr - Gly - Leu - Glu - Gly - Gly - Cys - Thr - Asp - Asp - Thr - Leu - Leu

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti- Adiponectin Receptor 1 (357-375) (Human) serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Alphabet_A_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Valeriy V. Lyzogubov, Ruslana G. Tytarenko, Nalini S. Bora, Puran S. Bora, Inhibitory role of adiponectin peptide I on rat choroidal neovascularization.
Biochim Biophys Acta. 2012 Aug;1823(8):1264-72. doi: 10.1016/j.bbamcr.2012.05.017. Epub 2012 May 23.

Williams et al. Adiponectin receptor expression is elevated in colorectal carcinomas but not in gastrointestinal stromal tumors.
Endocr Relat Cancer. 2008 Mar;15(1):289-99.