INSL 7, C Peptide (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat, Mouse brain and testis tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-INSL7, C Peptide (Human) Serum (Catalog No.:H-035-52)
Optimal Dilution	1: 500 (Overnight at 4^oC)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-035-52

Standard Size

100 µl

Sequence

Ser - Asp - Ile - Leu - Ala - His - Glu - Ala - Met - Gly - Asp - Thr - Phe - Pro - Asp - Ala - Asp - Ala - Asp - Glu - Asp - Ser - Leu - Ala - Gly - Glu - Leu - Asp - Glu - Ala - Met - Gly - Ser - Ser - Glu - Trp - Leu - Ala - Leu - Thr - Lys - Ser - Pro - Gln - Ala - Phe - Tyr - Arg - Gly - Arg - Pro - Ser - Trp - Gln - Gly - Thr - Pro - Gly - Val - Leu - Arg - Gly - Ser - Arg

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100ul of rabbit anti- INSL7, C Peptide (H) serum in the lyophilized form.

Recommended Dilution Factor

Immunohistochemistry (1:500) [Formalin/PFA-fixed paraffin-embedded sections]
Indirect ELISA (1: 6400)
Optimal dilutions/concentrations should be determined by the end user.

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