Urocortin III (Mouse) - Purified IgG Antibody

Catalog #	G-019-29
Standard Size	200 µg
Sequence	Phe - Thr - Leu - Ser - Leu - Asp - Val - Pro - Thr - Asn - Ile - Met - Asn - Ile - Leu - Phe - Asn - Ile - Asp - Lys - Ala - Lys - Asn - Leu - Arg - Ala - Lys - Ala - Ala - Ala - Asn - Ala - Gln - Leu - Met - Ala - Gln - Ile - NH2
Species	Mouse
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 145µl of distilled water and 55µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use.
Storage Condition	For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents	This vial contains 200µg of rabbit IgG in 145µl PBS (137mM NaCI, 2.7mM KCI, 4.3Mm Na₂HPO₄, 1.4mM KH₂PO₄, pH 7.2) in the lyophilized form.

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This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide; Urocortin III (Mouse) peptide can be detected at 1:12800 (0.078 µg/ml) in our ELISA system. We recommend that each laboratory determines an optimum titer for use in their particular assay system.

Applications:
Indirect ELISA: 1: 12800
Immunohistochemistry : 1:200