PYY (3-36) (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample Mouse and rat intestine tissue
Fixative 10% formalin
Embedding paraffin
Negative Control Pre-immuno serum
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)
Optimal Dilution 1:200~500 (1hour at RT)
Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification ABC (Vector) (1:400, 30 min)
Detection System HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Western Blot Analysis of PYY in rat intestine tissue
Tissue Sample	Rat intestine tissue homogenate
Electrophoresis	15% Tricine-PAGE gel
Negative control	No primary antibody
Blocking	1% BSA in TBST or 5% dry milk in TBST
Primary Antibody	Rabbit Anti-PYY (3-36) (H) purified IgG (Catalog No.:G-059-02)
Optimal Dilution	1:200 ( 5 ug /ml) (4 hour at RT, or overnight at 4⁰C)
Secondary Antibody	Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system	HRP
Substrate	SuperSignal Chemiluminescent Substrate

Catalog #

H-059-02

Standard Size

100 µl

Sequence

Ile - Lys - Pro - Glu - Ala - Pro - Gly - Glu - Asp - Ala - Ser - Pro - Glu - Glu - Leu - Asn - Arg - Tyr - Tyr - Ala - Ser - Leu - Arg - His - Tyr - Leu - Asn - Leu - Val - Thr - Arg - Gln - Arg - Tyr - NH2

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of Rabbit Anti- Peptide YY (3-36) (Human) Serum in the lyophilized form.

Recommended Dilution Factor

ABC: 1:200~500

Alphabet_P_

Please click here or click the reference tab on the top to see more articles that use this product.