AXOR12 (375-398) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Mouse Pancreas, Placenta
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact; Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Anti-AXOR12 (375-398) (Human) serum (H-048-61)
Optimal Dilution	1: 100
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector)
Detection system	HRP
Substrate	DAB (Sigma)
Counterstained	Hematoxylin

References for Protocol:
Ohtaki et al. Nature. 2001 May 31;411(6837):613-7.
Kotani et al. J Biol Chem. 2001 Sep 14;276(37):34631-6.
Muir et al. J Biol Chem. 2001 Aug 3;276(31):28969-75.

Catalog #

H-048-61

Standard Size

50 µl

Sequence

Ala - Gln - Lys - Pro - Gly - Ser - Ser - Gly - Leu - Ala - Ala - Arg - Gly - Leu - Cys - Val - Leu - Gly - Glu - Asp - Asn - Ala - Pro - Leu

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- AXOR12 (375-398) (Human) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Schmid et al. KiSS-1 overexpression as an independent prognostic marker in hepatocellular carcinoma: an immunohistochemical study
Virchows Arch. 2007 Feb;450(2):143-9.

Mead et al. Kisspeptins are novel potent vasoconstrictors in humans, with a discrete localization of their receptor, G protein-coupled receptor 54, to atherosclerosis-prone vessels.
Endocrinology. 2007 Jan;148(1):140-7.

Bilban et al. Kisspeptin-10, a KiSS-1/metastin-derived decapeptide, is a physiological invasion inhibitor of primary human trophoblasts.
J Cell Sci. 2004 Mar 15;117(Pt 8):1319-28.