Protocol for Immunohistochemistry:
Tissue Sample	Mouse brain or rat testis tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody (Pre-immuno serum)
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-INSL 5 (Mouse) Antiserum (Catalog No.:H-035-40)
Optimal Dilution	1:100~200 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec