Relaxin & Relaxin-Related Peptides

Insulin-like 3 Peptide (INSL 3)

Relaxin Evolution

GSS: Relaxin Like Peptide

Catalog #	035-27
Standard Size	100 µg
Sequence	A Chain:Ala - Ala - Ala - Thr - Asn - Pro - Ala - Arg - Tyr - Cys - Cys - Leu - Ser - Gly - Cys - Thr - Gln - Gln - Asp - Leu - Leu - Thr - Leu - Cys - Pro - Tyr B Chain: Pro - Thr - Pro - Glu - Met - Arg - Glu - Lys - Leu - Cys - Gly - His - His - Phe - Val - Arg - Ala - Leu - Val - Arg - Val - Cys - Gly - Gly - Pro - Arg - Trp - Ser - Thr - Glu - Ala Disulfide Bridge: A Chain:Cys10 - Cys15 A Chain Cys11 - B Chain Cys10 A Chain Cys24 - B Chain Cys22
Species	Human
M.W	6292.32
Mass Spec Analysis	Exhibits correct M.W.
Solubility	Before reconstitution, please allow 25 to 45 minutes for the lyophilized peptide to return to room temperature. Briefly vortex the Eppendorf tube to concentrate all material at the bottom of the tube. Gently open the cap and reconstitute the peptide to the desired concentration by adding a sterile, aqueous solution to the tube. Invert the tube 5 times by hand to ensure a homogeneous solution.
Storage Condition	Store peptide in DRY form at 0-5°C.
Appearance	White powder
Content	The contents of this vial have been accurately determined to contain 100µg of NET peptide.
HPLC Analysis	Purity ≥95%
Additional articles	For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this peptide:

Svendsen et al. Cooperative binding of insulin-like Peptide 3 to a dimeric relaxin family peptide receptor 2.
Endocrinology. 2008 Mar;149(3):1113-20.

El-Shewy et al. The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells
J Biol Chem. 2007 Sep 7;282(36):26150-7.

Kern et al. The Low-Density Lipoprotein Class A Module of the Relaxin Receptor (Leucine-Rich Repeat Containing GProtein Coupled Receptor 7): Its Role in Signaling and Trafficking to the Cell Membrane
Endocrinology. 2007 Mar;148(3):1181-94.

Bogatcheva et al. T222P mutation of the insulin-like 3 hormone receptor LGR8 is associated with testicular maldescent and hinders receptor expression on the cell surface membrane
Am J Physiol Endocrinol Metab. 2007 Jan;292(1):E138-44.

Bennett et al. Relaxin receptors in hepatic stellate cells and cirrhotic liver.
Biochem Pharmacol. 2007 Apr 1;73(7):1033-40.

Bennett et al. Relaxin receptor expression in hepatic stellate cells and in cirrhotic rat liver tissue.
Ann N Y Acad Sci. 2005 May;1041:185-9.

Liu et al. Relaxin-3/insulin-like peptide 5 chimeric peptide, a selective ligand for G protein-coupled receptor (GPCR)135 and GPCR142 over leucine-rich repeat-containing G protein-coupled receptor 7.
Mol Pharmacol. 2005 Jan;67(1):231-40.

Feng et al. Mutation analysis of INSL3 and GREAT/LGR8 genes in familial cryptorchidism.
Urology. 2004 Nov;64(5):1032-6.

Liu et al. Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein-coupled receptor GPCR135.
J Biol Chem. 2003 Dec 12;278(50):50754-64.

Liu et al. Identification of relaxin-3/INSL7 as a ligand for GPCR142.
J Biol Chem. 2003 Dec 12;278(50):50765-70.

Bogatcheva et al. GREAT/LGR8 is the only receptor for insulin-like 3 peptide.
Mol Endocrinol. 2003 Dec;17(12):2639-46.

For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.