R-SPONDIN-1

SFRP-5 and WNT-5a

Novel Paradigm of Metabolic Regulation

Catalog #	080-19A
Family	WNT
Standard Size	5 µg
Species	Human
Solubility	Before reconstitution, a brief spin is recommend to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a desired concentration.
Storage Condition	The lyophilized protein is stable for at least one year if stored at -80 degree C. Reconstituted protein is stable for at least four weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
Appearance	Lyophilized in sterile filtered solution of PBS.
HPLC Analysis	Greater than 90% as determined by SDS-PAGE analysis

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Description:
R-spondin-1 is a natural enhancer of the canonical WNT pathway. When used together with WNT proteins that activate the beta-catenin pathway, Rspondin-1 enhances the activity of WNT. It regulates Wnt/βcatenin by competing with the WNT antagonist DKK1 for binding to the WNT coreceptors, Kremen and LRP6, reducing their DKK1 mediated internalization.
Injection of recombinant RSpondin-1 into mouse causes activation of the βcatenin pathway and proliferation of intestinal crypt cells, which forms the basis for a clinical trial in amelioration of chemotherapy-induced colitis.
The Predicted MW of full-length R-Spondin-1 is 25. 6 kDa, and due to glycosylation it runs on SDSPAGE at ~39 kDa under reducing conditions.

Biological Activity:
The activity was determined by using a TCF reporter gene assay in cultured human cells. The EC50 ranges from 2 - 10 ng/ml in the presence of 10 ng/mL human WNT-3a. Activity in other assays should be determined by each individual setting.