CCK-33 (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Human colon & colon cancer tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit anti-CCK-33 (Human, Rat) antibody (Cat. No.: H-069-02)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-069-02

Standard Size

100 µl

Sequence

Lys - Ala - Pro - Ser - Gly - Arg - Met - Ser - Ile - Val - Lys - Asn - Leu - Gln - Asn - Leu - Asp - Pro - Ser - His - Arg - Ile - Ser - Asp - Arg - Asp - Tyr(SO3H) - Met - Gly - Trp - Met - Asp - Phe - NH2

Species

Human

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_C_