Metastin / Kiss-1 / GPR54 / AXOR12

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-048-59
Standard Size	96 wells
Sensitivity	Minimum Detectable Concentration = 35.4 pg/ml
Range	0-10000pg/ml
Linear Range	35.4-853 pg/ml
Cross Reactivity	Peptides %Cross-reactivity Metastin 1-54 (KiSS-1 (68-121))-Amide (Human)  100 RFRP-1 (Human) 100 RFRP-1 (Mouse,Rat) 54 Metastin (26-54)-Amide (Human)/ pGlu 26 25 NPAF (Human) 4 RFRP-3 (Human) 0 NPFF (NPSF) (Human) 0 FMRF- Amide 0
Sequence	Gly - Thr - Ser - Leu - Ser - Pro - Pro - Pro - Glu - Ser - Ser - Gly - Ser - Arg - Gln - Gln - Pro - Gly - Leu - Ser - Ala - Pro - His - Ser - Arg - Gln - Ile - Pro - Ala - Pro - Gln - Gly - Ala - Val - Leu - Val - Gln - Arg - Glu - Lys - Asp - Leu - Pro - Asn - Tyr - Asn - Trp - Asn - Ser - Phe - Gly - Leu - Arg - Phe - NH2
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	14.5-810 pg/ml
ED50	68.44 pg/ml
Sample Level	7.19 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.