Metastin / Kiss-1 / GPR54 / AXOR12

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-048-56
Standard Size	96 wells
Sensitivity	Minimum Detectable Concentration = 6.8 pg/ml
Range	0-10000 pg/ml
Linear Range	6.8-446 pg/ml
Cross Reactivity	Peptide % Cross-reactivity Metastin 1-54 (KiSS-1 (68-121))-Amide (Human)  100 Metastin 45-54-Amide (Human) 100 RFRP-1 (Human) 100 RFRP-1 (Mouse,Rat) 100 RFRP-3 (Human) 0 NPAF (Human) 4 NPFF (NPSF) (Human) 2 FMRF- Amide 2 KiSS-1 (110-119) Amide (Mouse) 2
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	16.7-553 pg/ml
ED50	60.82 pg/ml
Sample Level	5.5 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.