Endothelin and Endothelin-like Peptides

Preproendothelin-1: Placenta, Lung, Umbillical Vein (Human)

Endothelin-1 Precursor (ET-1) Sequence

Preproendothelin-1 (Porcine)

Normal Plasma Peptide/Protein Levels

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-023-01
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 12.9 pg/ml
Linear Range	17.8-185 pg/ml
Cross Reactivity	Peptide % Cross-reactivity Endothelin-1 (Human, Porcine, Rat, Mouse, Canine, Bovine, Rabbit, Monkey) 100 Endothelin-2 (Human, Porcine, Rat, Mouse, Canine, Bovine)  100 Endothelin-3 (Human, Porcine, Rat, Mouse, Canine, Bovine)  100 Big Endothelin-1 (Human) 16 Big Endothelin-2 (Human) 7 Angiotensin II (Human) 0 [Arg8]-Vasopressin 0
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	30.5-490 pg/ml
ED50	96.52 pg/ml
Sample Level	20 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.