Apelin - Research Abstracts, Antibody Stainings, Kits Standard Curve, Seqences, etc.

Normal Plasma Peptide/Protein Levels

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-057-23
Standard Size	96 well plate
Sensitivity	Minimum Detectable Concentration = 15.8 pg/ml
Range	0-10000 pg/ml
Linear Range	15.8-419 pg/ml
Cross Reactivity	Peptide  %Cross-reactivity   Apelin-12 (Human)  100  Apelin-13 (Human)  100  Apelin-13 (Rat)  100  Apelin-36 (Human)  100  Apelin-36 (Rat)  100  Alpha-ANP (Human)  0  ANP (25-56) (N-ANP)  0  ADM-52 (Human)  0  BNP-32 (Human)  0  CNP-22  0  Ghrelin (Human)  0  ET-1 (Human)  0  Bradykinin  0
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	16-384 pg/ml
ED50	61.8 pg/ml
Sample Level	14.5 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.