Orexins and Obesity

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-003-30
Standard Size	96 well plate
Sensitivity	Minimum Detectable Concentration = 42.5 pg/ml
Range	0-10000 pg/ml
Linear Range	42.5-480 pg/ml
Cross Reactivity	Peptide   % Cross-reactivity  Orexin A (Human, Bovine, Mouse, Rat)  100  Orexin A (16-33)  0  Orexin B (Human)  0  Agouti-Related Protein (83-132)-Amide (Human)  0  Neuropeptide (Human, Rat)  0  Alpha-MSH  0  Leptin (Human)  0
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	42.5-480 pg/ml
ED50	113.45 pg/ml
Sample Level	9.75 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.