Substance P

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-061-05
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 15 pg/ml
Range	0-10000 pg/ml
Linear Range	15-737 pg/ml
Cross Reactivity	Peptide % Cross-reactivity Substance P 100 Substance P (2-11) 100 Substance P (3-11) 100 Substance P (4-11) 100 Substance P (5-11) 100 Substance P (6-11) 5 Endothelin-1 (Human, Rat, Porcine,Mouse, Canine, Bovine)              < 0.5 Substance P (7-11)              < 0.01 Neuropeptide K (Porcine)              < 0.01 Neurokinin A              < 0.01 Neurokinin B 0 Somatostatin  0 Physalaemin 0
Sequence	Arg - Pro - Lys - Pro - Gln - Gln - Phe - Phe - Gly - Leu - Met - NH2
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	15-737 pg/ml
ED50	89.23 pg/ml
Sample Level	2 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.