Apelin - Research Abstracts, Antibody Stainings, Kits Standard Curve, Seqences, etc.

Normal Plasma Peptide/Protein Levels

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-057-15
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 20 pg/ml
Range	0-10000 pg/ml
Linear Range	20-480 pg/ml
Cross Reactivity	Peptide % Cross-reactivity Apelin-36 (Human) 100 Apelin-13 (Human) 100 Apelin-12 (Human) 100 Apelin-36 (Rat) 100 Alpha-ANP (Human) 0 ANP (25-56) (N-ANP) 0 ADM-52 (Human) 0 BNP-32 (Human) 0 CNP-22  0 Ghrelin (Human) 0 ET-1 (Human) 0 Bradykinin 0
Sequence	Leu - Val - Gln - Pro - Arg - Gly - Ser - Arg - Asn - Gly - Pro - Gly - Pro - Trp - Gln - Gly - Gly - Arg - Arg - Lys - Phe - Arg - Arg - Gln - Arg - Pro - Arg - Leu - Ser - His - Lys - Gly - Pro - Met - Pro - Phe
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	20-480 pg/ml
ED50	71.86 pg/ml
Sample Level	8.96 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.