AGRP (Human)

Canine Beta-Defensin CBD103

Normal Plasma Peptide/Protein Levels

Evidence of CNS

Protocol	Fluorescent EIA Kit (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	FEK-003-55
Standard Size	96 well
Sensitivity	Minimum Detectable Concentration = 28.4 pg/ml
Range	0-10000 pg/ml
Linear Range	28.4-298 pg/ml
Cross Reactivity	Peptide %Cross-reactivity Agouti-Related Protein Form C-NH2 (Human) 100 Agouti-Related Protein (83-132)-NH2 (Human) 100 Agouti-Related Protein (82-131)- NH2 (Mouse) 100 Leptin (Human) 0 Orexin A (Human, Mouse, Rat) 0 Orexin B (Human) 0 Neuropeptide Y (Human, Rat) 0 a-MSH 0 MCH (Human, Mouse, Rat) 0 CGRP (Rat) 0 CGRP (Human) 0
Sequence	Glu - Val - Leu - Asp - Leu - Gln - Asp - Arg - Glu - Pro - Arg - Ser - Ser - Arg - Arg - Cys - Val - Arg - Leu - His - Glu - Ser - Cys - Leu - Gly - Gln - Gln - Val - Pro - Cys - Cys - Asp - Pro - Cys - Ala - Thr - Cys - Tyr - Cys - Arg - Phe - Phe - Asn - Ala - Phe - Cys - Tyr - Cys - Arg - Lys - Leu - Gly - Thr - Ala - Met - Asn - Pro - Cys - Ser - Arg - Thr - NH2 -
Standard Curve Image

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Preliminary Plasma Levels

Sample Type	Human Extracted* Plasma (Pooled)
Linear Range **	57-575 pg/ml
ED50	135.07 pg/ml
Sample Level	11 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot.

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.