AGRP (71-132) Form C-Amide (Human)

Protocol

Warning: The Standard Protocol has been changed.
The Detection range of this RIA kit has been changed to
10-1280 pg/tube. Please note change in protocol booklet.

Assay Protocol (PDF)

Sample Preparation

Blood collection
Plasma extraction
Tissue extraction
CSF extraction

Catalog #

RK-003-55

Standard Size

125 RIA Tubes

Radioactivity

This kit contains 1.5 µCi of Iodine 125

Range

10-1280 pg/tube of 100 µl Standard Solution = 100-12,800 pg/ml

Lowest Detection Limit

176 pg/ml

Cross Reactivity

Peptide	% Cross-reactivity
Agouti-Related Protein Form C-NH₂ (Human)	100
Agouti-Related Protein (83-132)-NH₂ (Human)	100
Orexin A (Human, Mouse, Rat)	0
Orexin B (Human)	0
Neuropeptide Y (Human, Rat)	0
a-MSH	0

Sequence

Glu - Val - Leu - Asp - Leu - Gln - Asp - Arg - Glu - Pro - Arg - Ser - Ser - Arg - Arg - Cys - Val - Arg - Leu - His - Glu - Ser - Cys - Leu - Gly - Gln - Gln - Val - Pro - Cys - Cys - Asp - Pro - Cys - Ala - Thr - Cys - Tyr - Cys - Arg - Phe - Phe - Asn - Ala - Phe - Cys - Tyr - Cys - Arg - Lys - Leu - Gly - Thr - Ala - Met - Asn - Pro - Cys - Ser - Arg - Thr - NH2 - (Cyclized form, disulfide bridges undetermined)

Alphabet_A_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Shen et al. Plasma Agouti-Related Protein Level: A Possible Correlation with Fasted and Fed States in Humans and Rats
J Neuroendocrinol. 2002 Aug;14(8):607-10.

Dunbar et al. Changes in Hypothalamic Agouti-Related Protein (AGRP), but not α-MSH or Pro-Opiomelanocortin Concentrations in Dietary-Obese and Food-Restricted Rats
Exp Neurol. 2005 Jan;191(1):184-92.