Endothelin and Endothelin-like Peptides

Preproendothelin-1: Placenta, Lung, Umbillical Vein (Human)

Endothelin-1 Precursor (ET-1) Sequence

Normal Plasma Peptide/Protein Levels

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-023-10
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube of 100µl Standard Solution = 10-1280 pg/ml
Lowest Detection Limit	61.8 pg/ml
Cross Reactivity	Peptide  %Cross-reactivity   Big Endothelin-1 (Human)  100  Big Endothelin-1 (22-38) (Human)  100  Big Endothelin-1 (Rat)  7  Big Endothelin (22-39) (Porcine)  1  Big Endothelin (Porcine)  0  Endothelin-1 (Human, Rat, Porcine,  0     Mouse, Canine, Bovine)    Endothelin-2 (Human, Canine)  0  Endothelin-3 (Human, Rat)  0  BNP-32 (Porcine)  0  Sarafotoxin S6b  0
Sequence	Cys - Ser - Cys - Ser - Ser - Leu - Met - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp - Val - Asn - Thr - Pro - Glu - His - Val - Val - Pro - Tyr - Gly - Leu - Gly - Ser - Pro - Arg - Ser Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Frantz et al. Baseline and Serial Neurohormones in Patients With Congestive Heart Failure Treated With and Without Bucindolol: Results of the Neurohumoral Substudy of the Beta-Blocker Evaluation of Survival Study (BEST)
J Card Fail. 2007 Aug;13(6):437-44.

Frantz et al. Carvedilol therapy is associated with a sustained decline in brain natriuretic peptide levels in patients with congestive heart failure.
Am Heart J. 2005 Mar;149(3):541-7.

Kocík et al. The effect of cyclosporin A on the level of big endothelin in patients one year after orthotopic heart transplantation.
Transpl Int. 2004 Feb;17(2):65-70.

Grantham et al. Modulation of functionally active endothelin-converting enzyme by chronic neutral endopeptidase inhibition in experimental atherosclerosis.
Circulation. 2000 Apr 25;101(16):1976-81.

for plasma and tissue: Grantham JA. - Circulation. 2000;101:1976