Endothelin and Endothelin-like Peptides

Preproendothelin-1: Placenta, Lung, Umbillical Vein (Human)

Endothelin-1 Precursor (ET-1) Sequence

Preproendothelin-1 (Porcine)

Normal Plasma Peptide/Protein Levels

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-023-01
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube in 100µl of Standard Solution = 10-1280 pg/ml
Lowest Detection Limit	29.1 pg/ml
Cross Reactivity	Peptide %Cross-reactivity   Endothelin-1 (Human, Rat, Mouse, Porcine, Bovine, Canine, Rabbit, Monkey)  100  Endothelin-2 (Human, Canine)  100  Big Endothelin-2 (Human)  100  Big Endothelin-1 (Human)   76  Endothelin-3 (Human, Rat, Porcine)    0  Angiotensin II (Human)    0  Urotensin II (Human)    0
Sequence	Cys - Ser - Cys - Ser - Ser - Leu - Met - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Zhang et al. Pentoxifylline attenuation of experimental hepatopulmonary syndrome.
J Appl Physiol. 2007 Mar;102(3):949-55.

Lin et al. 17beta-estradiol inhibits endothelin-1 production and attenuates cerebral vasospasm after experimental subarachnoid hemorrhage.
Exp Biol Med (Maywood). 2006 Jun;231(6):1054-7.

Wang et al. Regulation of the rat brain endothelin system by endogenous beta-endorphin.
Peptides. 2006 Apr;27(4):769-74.

Forni et al. Expression of endothelin-1 system in a pig model of endotoxic shock.
Regul Pept. 2005 Nov;131(1-3):89-96.

Luo et al. Cholangiocyte endothelin 1 and transforming growth factor beta1 production in rat experimental hepatopulmonary syndrome.
Gastroenterology. 2005 Aug;129(2):682-95.

Li et al. Ghrelin blunted vascular calcification in vivo and in vitro in rats.
Regul Pept. 2005 Jul 15;129(1-3):167-76.

Ariza et al. Effects of magnesium sulfate on lipid peroxidation and blood pressure regulators in preeclampsia.
Clin Biochem. 2005 Feb;38(2):128-33.

Luo et al. ET-1 and TNF-alpha in HPS: analysis in prehepatic portal hypertension and biliary and nonbiliary cirrhosis in rats.
Am J Physiol Gastrointest Liver Physiol. 2004 Feb;286(2):G294-303.

Liang et al. Triiodothyronine increases brain natriuretic peptide (BNP) gene transcription and amplifies endothelin-dependent BNP gene transcription and hypertrophy in neonatal rat ventricular myocytes.
J Biol Chem. 2003 Apr 25;278(17):15073-83.

Qi et al. Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells
Chin. Sci Bul. 2002 Sep 17;47(17):1457-61. (PDF)

Liu et al. Biliary cyst fluid from common bile duct-ligated rats stimulates endothelial nitric oxide synthase in pulmonary artery endothelial cells: a potential role in hepatopulmonary syndrome.
Hepatology. 2001 Mar;33(3):722-7.

Givertz et al. Acute endothelin A receptor blockade causes selective pulmonary vasodilation in patients with chronic heart failure.
Circulation. 2000 Jun 27;101(25):2922-7.

Grantham et al. Modulation of functionally active endothelin-converting enzyme by chronic neutral endopeptidase inhibition in experimental atherosclerosis.
Circulation. 2000 Apr 25;101(16):1976-81.

Deng et al. Intra-atrial communication and control of atrial natriuretic factor (ANF) release.
Can J Physiol Pharmacol. 2000 Jan;78(1):1-6.

Friedrich et al. Contribution of the endothelin system to the renal hypoperfusion associated with experimental congestive heart failure.
J Cardiovasc Pharmacol. 1999 Oct;34(4):612-7.

Liang F. - J. Biol. Chem. - Apr 2003; 278: 15073 - 15083 / for plasma and tissue: Grantham JA. - Circulation. 2000;101:1976