Brain Natriuretic Peptides (BNP)

Normal Plasma Peptide/Protein Levels

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-011-22
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube in 100ul of Standard Solution = 10-1280 pg/ml
Lowest Detection Limit	43.7 pg/ml
Cross Reactivity	Peptide %Cross-reactivity  BNP-32 (Canine)  100  BNP-32 (Porcine)  100  BNP-26 (Porcine)  100  BNP-32 (Human)  0  BNP-32 (Rat)  0  BNP-45 (Rat)  0  a-ANP (1-28) (Human, Canine)  0  CNP-22 (Human, Porcine, Rat)  0  Endothelin-1 (Human, Bovine, Canine, Mouse, Porcine, Rat)  0
Sequence	Ser - Pro - Lys - Met - Met - His - Lys - Ser - Gly - Cys - Phe - Gly - Arg - Arg - Leu - Asp - Arg - Ile - Gly - Ser - Leu - Ser - Gly - Leu - Gly - Cys - Asn - Val - Leu - Arg - Lys - Tyr

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Schellenberg et al. Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog.
Vet J. 2008 Oct;178(1):85-90.

Oyama et al. Prospective screening for occult cardiomyopathy in dogs by measurement of plasma atrial natriuretic peptide, B-type natriuretic peptide, and cardiac troponin-I concentrations
Am J Vet Res. 2007 Jan;68(1):42-7.

Donker et al. Impact of acute and enduring volume overload on mechanotransduction and cytoskeletal integrity of canine left ventricular myocardium
Am J Physiol Heart Circ Physiol. 2007 May;292(5):H2324-32.

Tsuruda et al. Brain natriuretic Peptide is produced in cardiac fibroblasts and induces matrix metalloproteinases.
Circ Res. 2002 Dec 13;91(12):1127-34.

Tsuruda T. Circ. Res. - Dec 2002; 91: 1127 - 1134