Brain Natriuretic Peptides (BNP)

Normal Plasma Peptide/Protein Levels

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-011-17
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube in 100ul of Standard Solution = 10-1280 pg/ml
Lowest Detection Limit	54 pg/ml
Cross Reactivity	Peptide: %Cross-reactivity  BNP-45 (Rat)  100  BNP-32 (Rat)  100  BNP-45 (Mouse)  41  BNP-32 (Human)  0  BNP-32 (Porcine)  0  BNP-26 (Porcine)  0  BNP-32 (Canine)  0  ANP (Rat)  0  a-ANP (1-28) (Human, Porcine)  0  Endothelin-1     (Human, Bovine, Canine, Mouse, Rat, Porcine)    0  Angiotensin II (Human)  0
Sequence	Ser - Gln - Asp - Ser - Ala - Phe - Arg - Ile - Gln - Glu - Arg - Leu - Arg - Asn - Ser - Lys - Met - Ala - His - Ser - Ser - Ser - Cys - Phe - Gly - Gln - Lys - Ile - Asp - Arg - Ile - Gly - Ala - Val - Ser - Arg - Leu - Gly - Cys - Asp - Gly - Leu - Arg - Leu - Phe ~Disulfide bridge between Cys23 & Cys39

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Jiang et al. Changes in production and metabolism of brain natriuretic peptide in rats with myocardial necrosis.
Eur J Pharmacol. 2005 Jan 10;507(1-3):153-62.

Liang et al. Triiodothyronine increases brain natriuretic peptide (BNP) gene transcription and amplifies endothelin-dependent BNP gene transcription and hypertrophy in neonatal rat ventricular myocytes.
J Biol Chem. 2003 Apr 25;278(17):15073-83.

Liang F. - J. Biol. Chem. - Apr 2003; 278: 15073 - 15083