ANP (Atrial Natriuretic Peptide)

Normal Plasma Peptide/Protein Levels

Protocol	Assay Protocol (PDF)
Sample Preparation	Blood collection Plasma extraction Tissue extraction CSF extraction
Catalog #	RK-005-24
Standard Size	125 RIA tubes
Radioactivity	This kit contains 1.5 µCi of Iodine 125
Range	1-128 pg/tube of 100µl Standard Solution = 10-1280 pg/ml
Lowest Detection Limit	47.4 pg/ml
Cross Reactivity	Peptide % Cross-reactivity  ANP (Rat, Mouse)  100  a-ANP (1-28) (Human, Ovine, Canine)  100  b-ANP (Human)  100  Urodilatin  100  BNP-26 (Porcine)  0  Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)  0  BNP-32 (Human)  0  BNP-45 (Rat)  0  CNP-22 (Human, Porcine, Rat)  0
Sequence	Ser - Leu - Arg - Arg - Ser - Ser - Cys - Phe - Gly - Gly - Arg - Ile - Asp - Arg - Ile - Gly - Ala - Gln - Ser - Gly - Leu - Gly - Cys - Asn - Ser - Phe - Arg - Tyr

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

 

Christoph Birner, Coskun Ulucan, Mona Bratfisch, et al, Antihypertrophic effects of combined inhibition of the renin-angiotensin system (RAS) and neutral endopeptidase (NEP) in progressive, tachycardia-induced experimental heart failure. 
Naunyn Schmiedebergs Arch Pharmacol. 2012 Nov;385(11):1117-25. doi: 10.1007/s00210-012-0791-6. Epub 2012 Aug 16. 

Heimeier et al. The response of the natriuretic peptide system to water deprivation in the desert rodent, Notomys alexis
Comp Biochem Physiol A Mol Integr Physiol. 2006 Feb;143(2):193-201.

Fredj et al. Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
J Cell Physiol. 2005 Aug;204(2):428-36. 

Fredj et al. Interactions between cardiac cells enhance cardiomyocyte hypertrophy and increase fibroblast proliferation.
J Cell Physiol. 2005 Mar;202(3):891-9.

Chandrasekar et al. Interleukin-18 is a pro-hypertrophic cytokine that acts through a phosphatidylinositol 3-kinase-phosphoinositide-dependent kinase-1-Akt-GATA4 signaling pathway in cardiomyocytes.
J Biol Chem. 2005 Feb 11;280(6):4553-67. 

Hunt et al. Hypotension and bradycardia during caloric restriction in mice are independent of salt balance and do not require ANP receptor
Am J Physiol Heart Circ Physiol. 2004 Oct;287(4):H1446-51.

Tran et al. Upregulation of corin gene expression in hypertrophic cardiomyocytes and failing myocardium.
Am J Physiol Heart Circ Physiol. 2004 Oct;287(4):H1625-31.

Francis et al. Progression of heart failure after myocardial infarction in the rat.
Am J Physiol Regul Integr Comp Physiol. 2001 Nov;281(5):R1734-45.

Leuranguer et al. T-type calcium currents in rat cardiomyocytes during postnatal development: contribution to hormone secretion.
Am J Physiol Heart Circ Physiol. 2000 Nov;279(5):H2540-8.

Tamura et al. Changes in atrial natriuretic peptide concentration and expression of its receptors after pneumonectomy in the rat.
Clin Sci (Lond). 2000 Oct;99(4):343-8.

Carlson et al. Hepatic denervation does not affect plasma vasopressin response to intragastric hypertonic saline in conscious rats.
Am J Physiol. 1999 Jul;277(1 Pt 1):E161-7.

Leuranguer - AJP - Heart & Circul. Phys. - Vol. 279 - Issue 5 - H2540-H2548 - November 2000

 

Leuranguer - AJP - Heart & Circul. Phys. - Vol. 279 - Issue 5 - H2540-H2548 - November 2000