Beta Defensins

Anti-Microbial Peptides

Canine Beta-Defensin CBD103

Catalog #	G-072-53
Standard Size	200 µg
Sequence	Asp - His - Tyr - Asn - Cys - Val - Ser - Ser - Gly - Gly - Gln - Cys - Lys - Tyr - Ser - Ala - Cys - Pro - Ile - Phe - Thr - Lys - Ile - Gln - Gly - Thr - Cys - Tyr - Arg - Gly - Lys - Ala - Lys - Cys - Cys - Lys ~ Disulfide bridges between Cys1 & Cys5, Cys2 & Cys4 and Cys3 & Cys6
Species	Human
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 90µl of distilled water and 110µl PBS for the equivalent of undiluted antibody, immediately before use.
Storage Condition	For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents	This vial contains 200µg of rabbit IgG in 90µl PBS (137mM NaCI, 2.7mM KCI, 4.3mM Na2HPO4, 1.4mM KH2PO4, pH 7.5) in the lyophilized form.
Cross Reactivity	Peptide  % Cross-reactivity  b-Defensin 1 (Human)  100  b-Defensin 2 (Human)  0  b-Defensin 8 (Mouse)  0  LL37 (Human)  0  TNF-a (Human)  0

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This product was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, β-Defensin 1 (Human) can be detected with this IgG (1:6400, 0.156µg/ml) in ELISA. We recommend that each laboratory determine an optimum titer for use in their particular assay system.