This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, apelin-12 (human, bovine) can be detected with this IgG (1:3200, 0.312ng/ml) in ELISA. We recommend that each laboratory determine an optimum titer for use in their particular assay system.
Links to publications that use this antibody:
Zhong JC, Huang DY, Liu GF, et al. Effects of all-trans retinoic acid on orphan receptor APJ signaling in spontaneously hypertensive rats. Cardiovascular Research. 2005;65(3):743-50.
Atluri P, Morine KJ, Liao GP, et al. Ischemic heart failure enhances endogenous myocardial apelin and APJ receptor expression. Cellular & Molecular Biology Letters. 2007;12(1):127-38.
Jia YX, Pan CS, Zhang J, et al. Apelin protects myocardial injury induced by isoproterenol in rats. Regulatory Peptides. 2006;133(1-3):147-54.