Ghrelin (Rat, Mouse) - Purified IgG Antibody

Catalog #

G-031-31

Standard Size

200 µg

Sequence

Gly - Ser - Ser(Octanoyl) - Phe - Leu - Ser - Pro - Glu - His - Gln - Lys - Ala - Gln - Gln - Arg - Lys - Glu - Ser - Lys - Lys - Pro - Pro - Ala - Lys - Leu - Gln - Pro - Arg

Species

Rat, Mouse

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 80µl of distilled water and 120µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Contents

Each vial contains 200µg of rabbit anti-Ghrelin (Rat Mouse) IgG in 65µl PBS (137 mM NaCL, 2.7 mM KCL, 4.3 mM Na₂HPO₄, 1.4 mM KH₂PO₄, pH 7.2 ) lyophilized form.

Recommended Dilution Factor

This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, Ghrelin (Rat Mouse) can be detected with this IgG (1:6400, 0.156ug/ml) in our ELISA system. We recommend that each laboratory to determine an optimum titer for use in its particular assay system.

Cross Reactivity

*Peptide*	*% Cross-reactivity*
Ghrelin (Rat Mouse)	100
Ghrelin (Human)	100
Ghrelin (Des-Octanoyl-Ser3) (Human)	100
Ghrelin (Des-Octanoyl-Ser3) (Rat Mouse)	100
Ghrelin (17-28) (Human, Rat)	100
Ghrelin (Canine)	100
Ghrelin C-terminal Hexapeptide (Human Rat)	3
Ghrelin (Porcine)	0
Ghrelin (1-14) (Human)	0
Ghrelin (1-5)-NH2 (Human Rat)	0
Ghrelin (1-5)- NH2 (Des-Octanyl³) (Human Rat)	0
Ghrelin (1-14) (Des-Octanoyl³) (Human)	0
Secretin (Human)	0
VIP (Human Rat Porcine)	0
Prolactin-Releasing Peptide-31 (Rat)	0
Prolactin-Releasing Peptide-31 (Human)	0
Galanin (Rat)	0
Galanin (Human)	0
GHRF (Rat)	0
GHRF (Human)	0
NPY (Human, Rat)	0
Orexin A (Human Rat Mouse)	0

Alphabet_G_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Solomon et al. Peripheral ghrelin interacts with orexin neurons in glucostatic signalling.
Regul Pept. 2007 Dec 4;144(1-3):17-24.

Solomon et al. Peripheral ghrelin participates in the glucostatic signaling mediated by the ventromedial and lateral hypothalamus neurons.
Peptides. 2006 Jul;27(7):1607-15.