Endothelin-2 (ET-2) (Human, Canine) - Purified IgG Antibody

Catalog #

G-023-13

Standard Size

400 µg

Sequence

Cys - Ser - Cys - Ser - Ser - Trp - Leu - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp

Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11

Species

Human, Canine

Host

Rabbit

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Contents

Each vial contains 400µg of rabbit IgG in lyophilized form, which was purified from whole serum by Protein A affinity.

Cross Reactivity

*Peptide*	*% Cross-reactivity*
Endothelin 2 (Human, Canine)	100
Endothelin 1 (Human, Rat, Porcine, Mouse, Canine, Bovine)	100
Endothelin-3 (Human, Porcine, Rabbit, Rat)	100
Big Endothelin 1 (Human)	100
Big Endothelin 22-38 (Human)	0
Sarafotoxin S6b	0
BNP-26 (Porcine)	0
Angiotensin II (Human)	0
a-ANP (Human, Canine, Porcine, Bovine)	0

Alphabet_E_

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Upon reconstitution with 200µl of distilled water, the solution contains 0.1M PBS (pH 7.4) and 0.02% thimerasol. The 0.8µg/ml final concentration of IgG will give about 40% total binding in our RIA system. We recommend that each laboratory determine an optimum titer for use in its particular assay system.