Urocortin (Rat, Mouse) - Purified IgG Antibody

Catalog #

G-019-15

Standard Size

400 µg

Sequence

Asp - Asp - Pro - Pro - Leu - Ser - Ile - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Thr - Leu - Leu - Glu - Leu - Ala - Arg - Thr - Gln - Ser - Gln - Arg - Glu - Arg - Ala - Glu - Gln - Asn - Arg - Ile - Ile - Phe - Asp - Ser - Val - NH2

Species

Rat, Mouse

Host

Rabbit

Reconstitution

For consistent and reproducible results, after rehydration, store the aliquots at -20°C (or below). Do not refreeeze any unused portions.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Contents

Each vial contains @400µg of rabbit IgG in lyophilized form.

Cross Reactivity

*Peptide*	*% Cross-reactivity*
Urocortin (Rat)	100
Urocortin (Human)	100
Cortistatin	<1
CRF (Human, Rat)	0
rMCH	0
LH-RH	0
NPY (Human, Rat)	0
Somatostatin	0

Alphabet_U_

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This was purified from whole serum by Protein A affinity column.(8µg/ml) final concentration of IgG will give about 40% total binding in our RIA system using ¹²⁵I-[Tyr⁰]-Urocortin (Rat) as tracer. We recommend that each laboratory determine an optimum titer for use in its particular assay system.