Human Pancreas tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 10 min (Steam)*
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Amylin (Human) Purified IgG (Catalog No: H-017-03)
Optimal Dilution
1:200 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
* Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
