BNP-32 (Rat) - Purified IgG Antibody

Catalog #

G-011-14

Standard Size

400 µg

Sequence

Asn - Ser - Lys - Met - Ala - His - Ser - Ser - Ser - Cys - Phe - Gly - Gln - Lys - Ile - Asp - Arg - Ile - Gly - Ala - Val - Ser - Arg - Leu - Gly - Cys - Asp - Gly - Leu - Arg - Leu - Phe

Disulfide Bridge: Cys10 - Cys26

Species

Rat

Host

Rabbit

Reconstitution

For consistent and reproducible results, dissolve the content with 135µl distilled water and 265µl PBS, then dilute to any desire buffer. Do not refreeze any unused portions.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Contents

This vial contains 400µg of rabbit IgG in 135µl (137 mM NCI, 2.7 mM KCI, 4.3 mM Na₂HPO₄, 1.4 mM KH₂PO₄, pH 7.5) in the lyophilized form.

Cross Reactivity

*Peptide*	*%Cross-reactivity*
BNP-32 (Rat)	100
BNP-45 (Rat)	100
BNP-32 (Porcine)	0
BNP-26 (Porcine)	0
BNP-32 (Human)	0
BNP-32 (Canine)	0
ANF (Rat, Mouse, Rabbit)	0
a-ANP 1-28 (Human, Canine, Porcine, Bovine)	0
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)	0
Angiotensin II (Human)	0

Alphabet_B_

Please click here or click the reference tab on the top to see more articles that use this product.

This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, BNP-32 (rat) can be detected with this IgG (1: 31K) in ELISA. We recommend that each laboratory determines an optimum titer for use in their particular assay system.

Links to the publications that use this antibody:

Ogawa T, De bold AJ. Uncoordinated regulation of atrial natriuretic factor and brain natriuretic peptide in lipopolysaccharide-treated rats.
Biomarkers. 2012;17(2):140-9.