Brain Natriuretic Peptides (BNP)

Normal Plasma Peptide/Protein Levels

Catalog #	G-011-03
Standard Size	400 µg
Sequence	Ser - Pro - Lys - Met - Val - Gln - Gly - Ser - Gly - Cys - Phe - Gly - Arg - Lys - Met - Asp - Arg - Ile - Ser - Ser - Ser - Ser - Gly - Leu - Gly - Cys - Lys - Val - Leu - Arg - Arg - His Disulfide Bridge: Cys10 - Cys26
Species	Human
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 112µl of distilled water and 288µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use.
Storage Condition	For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents	Each vial contains 400ug in 112ul PBS (137 mM NaCL, 2.7 mM KCL, 4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH 7.2) in the lyophilized form.
Cross Reactivity	Peptide % Cross-reactivity   BNP-32 (Human)  100  BNP-32 (Rat)   < 0.04  BNP-45 (Rat)  0  BNP-26 (Porcine)  0  a-ANP (1-28) (Human, Canine)  0  Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)   0  Angiotensin II (Human)  0  VIP (Human, Rat, Porcine)  0
Additional articles	This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, BNP-32 (H) can be detected with this IgG at 1:1600 (0.625 µg/ml) in ELISA. We recommend that each laboratory determine an optimum titer for use in its particular assay system.

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Hawkridge et al. Quantitative mass spectral evidence for the absence of circulating brain natriuretic peptide (BNP-32) in severe human heart failure.
Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17442-7.

Matsuura et al. Surface electrochemical enzyme immunoassay for the highly sensitive measurement of B-type natriureric peptide
Chem. Sens. Actuators B. 2005 July;108(1-2):R603-607.

This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, BNP-32 (H) can be detected with this IgG at 1:1600 (0.625 µg/ml) in ELISA. We recommend that each laboratory determine an optimum titer for use in its particular assay system.