CART, Research Abstracts, Photomicrographs & Sequences

Cocaine and Amphetamine-Regulated Transcript (CART) Protein Precursor Schematic (Human)

CART Preprohormone Schematic (Rat)

Prepro-CART Sequence (Rat)

Catalog #	G-003-61
Standard Size	200 µg
Sequence	Lys - Tyr - Gly - Gln - Val - Pro - Met - Cys - Asp - Ala - Gly - Glu - Gln - Cys - Ala - Val - Arg - Lys - Gly - Ala - Arg - Ile - Gly - Lys - Leu - Cys - Asp - Cys - Pro - Arg - Gly - Thr - Ser - Cys - Asn - Ser - Phe - Leu - Leu - Lys - Cys - Leu [Disulfide Bridge: Cys8 - Cys26 Cys14 - Cys34 and Cys28 - Cys41]
Species	Human, Rat, Mouse
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 90µl of distilled water and 110µl of PBS for the equivalent of undiluted antibody, immediately before use. Do not refreeze any unused portions.
Storage Condition	For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents	This vial contains 200µg of rabbit IgG in 90µl PBS (137mM NaCI, 2.7mM KCI, 4.3mM Na2HPO4, 1.4mM KH2PO4, pH7.2) in the lyophilized form.
Cross Reactivity	Peptide   %Cross-reactivity  CART (61-102) (Human, Rat)  100  CART (55-102) (Rat)  100  Agouti-Related Protein 83-132-NH2 (Human)  2  Leptin (Human)  0  Neuropeptide (Human, Rat)  0  a-MSH  0  Orexin A (Human, Mouse, Rat)  0  Orexin B (Human)  0
Image
Additional articles	This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide CART (61-102) (human, Rat) can be detected with this IgG (1:6400, 0.156µg/ml) in a ELISA system. We recommend that each laboratory determines an optimum titer for use in their particular assay system.

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this kit:

Xu et al. Role of cocaine- and amphetamine-regulated transcript in estradiol-mediated neuroprotection
Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14489-94.

This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide CART (61-102) (human, Rat) can be detected with this IgG (1:6400, 0.156µg/ml) in a ELISA system. We recommend that each laboratory determines an optimum titer for use in their particular assay system.