AUF-1 (151-199) (Human) - Purified IgG Antibody

Catalog #	G-001-50
Standard Size	200 µg
Sequence	Ala - Lys - Ala - Met - Lys - Thr - Lys - Glu - Pro - Val - Lys - Lys - Ile - Phe - Val - Gly - Gly - Leu - Ser - Pro - Asp - Thr - Pro - Glu - Glu - Lys - Ile - Arg - Glu - Tyr - Phe - Gly - Gly - Phe - Gly - Glu - Val - Glu - Ser - Ile - Glu - Leu - Pro - Met - Asp - Asn - Lys - Thr
Species	Human
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 200µl of distilled water and 200µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use. Do not refreeze any unused portions.
Storage Condition	For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents	This vial contains 200µg of rabbit IgG in 200µl PBS (137mM NaCI, 2.7mM KCI, 4.3mM Na₂HPO₄, 1.4mM KH₂PO₄, pH7.4) in the lyophilized form.
Image	Western Blot Analysis for human RNA Binding Proteins (hnRNPs) Western blot analysis for hnRNPs (nuclear and cytoplasmic) after infection (48hr) and actinomycine D treatment (24hr, 10µg/ml). VHL is expressed in AdVHL-infected 786-0 cells and downregulated in ActD-treated 786-0 cells.
Additional articles	This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, AUF (151-199) (human) can be detected with this IgG (1:4000, 0.25µg/ml) in ELISA. We recommend that each laboratory determines an optimum titer for use in their particular assay system.

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Links to publications that use this antibody:

Navarro-Yubero et al. Neuroserpin is post-transcriptionally regulated by thyroid hormone.
Brain Res Mol Brain Res. 2004 Apr 7;123(1-2):56-65.

Ambrosino et al. Negative feedback regulation of MKK6 mRNA stability by p38alpha mitogen-activated protein kinase.
Mol Cell Biol. 2003 Jan;23(1):370-81.This was purified from whole serum by Protein A affinity column. The HPLC purified standard peptide, AUF (151-199) (human) can be detected with this IgG (1:4000, 0.25µg/ml) in ELISA. We recommend that each laboratory determines an optimum titer for use in their particular assay system.