Adipo R1 (41-65) / Adiponectin Receptor Type 1 (41-65) (Human)

多肽

标记多肽

多肽激素文库

抗体

免疫组化抗体
纯化免疫球蛋白

Other Purified antibodies
抗体标记

免疫试剂盒

生物标志物阵列

Adipo R1 (41-65) / Adiponectin Receptor Type 1 (41-65) (Human) - Purified IgG Antibody

Catalog#	Standard size	Price	Order
G-001-45	200 µg	$450.00

Catalog #

G-001-45

Standard Size

200 µg

Sequence

Val - Ile - Ala - Asn - Pro - Pro - Lys - Ala - Glu - Glu - Glu - Gln - Thr - Cys - Pro - Val - Pro - Gln - Glu - Glu - Glu - Glu - Glu - Val - Arg

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 85µl of distilled water and 115µl PBS (or 50% glycerol in PBS) for the equivalent of undiluted antibody, immediately before use.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Contents

This vial contains 200µg of rabbit IgG in 85µl PBS (137mM NaCI, 2.7mM KCI, 4.3mM Na₂HPO₄, 1.4mM KH₂PO₄, pH7.4) in the lyophilized form.

Recommended Dilution Factor

Western blot analysis: 1: 200
Immunohistochemistry: 1: 100~200

Image

Protocol for Immunohistochemistry
Tissue Sample	Rat muscle tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody (Pre-immuno serum)
Pretreatment	Target retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Adiponectin Receptor 1 (41-65) (Human) Purified IgG (Catalog No.:G-001-45)
Optimal Dilution	1:100~200 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

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