Visfatin / PBEF Isoform I, His (Rec.) (Mouse)

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Protocol for Immunohistochemistry:
Tissue Sample	Rat or Mouse Visceral white fat tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Visfatin Isoform I, His, (Rec.) (Mouse) (Catalog No.:G-003-91)
Optimal Dilution	1:1000 ~ 2000 (Overnight at 4^oC)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (sigma), 30min
Counterstained	Hematoxylin, 30Sec

Catalog #

G-003-91

Standard Size

100 µg

Sequence

Mouse PBEF Isoform I, 6 x His on N - Terminal.

Species

Mouse

Host

Rabbit

Reconstitution

Reconstitute with 100 ul distill water or PBS.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Content

100 ul NET Rabbit serum in Lyophilized powder without NaN3 and carry protein.

Recommended Dilution Factor

Indirect ELISA: 1: 6400
Immunohistochemistry:1: 1000~2000

Cross Reactivity

It is cross reactive with Mouse Visfatin/PBEF Isoform I, His, (Rec.). It was tested in immunohistochemistry in mouse and rat visceral white fat tissues. Not yet tested in other species.

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